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(A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed <t>at</t> <t>7d</t> post-ACLR. (B-E) Whole blood neutrophil counts for (B) males and (C) females at 7d post-ACLR (n=4 per sex/treatment). Whole blood monocyte counts for (D) males and (E) females at 7d post-ACLR (n=4 per sex/treatment). Welch’s t-test run for (B-E). (F) Knee withdrawal threshold of the ACLR limb and (G) ACLR/contralateral knee withdrawal threshold ratio at baseline and 7d post-ACLR (n=4 per sex/treatment). (H) Representative images from Prosense680 <t>NIR</t> molecular imaging. (I) ProSense680 signal intensity quantification in the ACLR limb at 7d (n=4 per sex/treatment). Histopathologic scoring of (J) total joint PTOA severity and (K) total joint synovitis severity (n=4 per sex/treatment). Statistical analyses for F-K used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.
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(A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed <t>at</t> <t>7d</t> post-ACLR. (B-E) Whole blood neutrophil counts for (B) males and (C) females at 7d post-ACLR (n=4 per sex/treatment). Whole blood monocyte counts for (D) males and (E) females at 7d post-ACLR (n=4 per sex/treatment). Welch’s t-test run for (B-E). (F) Knee withdrawal threshold of the ACLR limb and (G) ACLR/contralateral knee withdrawal threshold ratio at baseline and 7d post-ACLR (n=4 per sex/treatment). (H) Representative images from Prosense680 <t>NIR</t> molecular imaging. (I) ProSense680 signal intensity quantification in the ACLR limb at 7d (n=4 per sex/treatment). Histopathologic scoring of (J) total joint PTOA severity and (K) total joint synovitis severity (n=4 per sex/treatment). Statistical analyses for F-K used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.
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Image Search Results


(A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed at 7d post-ACLR. (B-E) Whole blood neutrophil counts for (B) males and (C) females at 7d post-ACLR (n=4 per sex/treatment). Whole blood monocyte counts for (D) males and (E) females at 7d post-ACLR (n=4 per sex/treatment). Welch’s t-test run for (B-E). (F) Knee withdrawal threshold of the ACLR limb and (G) ACLR/contralateral knee withdrawal threshold ratio at baseline and 7d post-ACLR (n=4 per sex/treatment). (H) Representative images from Prosense680 NIR molecular imaging. (I) ProSense680 signal intensity quantification in the ACLR limb at 7d (n=4 per sex/treatment). Histopathologic scoring of (J) total joint PTOA severity and (K) total joint synovitis severity (n=4 per sex/treatment). Statistical analyses for F-K used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.

Journal: bioRxiv

Article Title: Acute immune modulation with poly-salicylic acid particles ameliorates pain and structural damage in post-traumatic osteoarthritis

doi: 10.1101/2025.11.21.689790

Figure Lengend Snippet: (A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed at 7d post-ACLR. (B-E) Whole blood neutrophil counts for (B) males and (C) females at 7d post-ACLR (n=4 per sex/treatment). Whole blood monocyte counts for (D) males and (E) females at 7d post-ACLR (n=4 per sex/treatment). Welch’s t-test run for (B-E). (F) Knee withdrawal threshold of the ACLR limb and (G) ACLR/contralateral knee withdrawal threshold ratio at baseline and 7d post-ACLR (n=4 per sex/treatment). (H) Representative images from Prosense680 NIR molecular imaging. (I) ProSense680 signal intensity quantification in the ACLR limb at 7d (n=4 per sex/treatment). Histopathologic scoring of (J) total joint PTOA severity and (K) total joint synovitis severity (n=4 per sex/treatment). Statistical analyses for F-K used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.

Article Snippet: At harvest, either 7d or 28d post-ACLR, mice were euthanized, skinned, and NIR imaging (Pearl Impulse, LI-COR, Lincoln, NE, United States) was performed (7d: n=4 per sex/treatment, 28d: n=8 females per treatment).

Techniques: Imaging

(A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed at 28d post-ACLR. (B-C) Knee withdrawal threshold of the ACLR limb (B) and ACLR/contralateral knee withdrawal threshold ratio (C) at baseline and 28d post-ACLR (n=8 per treatment). (D) Signal intensity quantification from ProSense680 NIR molecular imaging in the ACLR limb at 28d (n=8 per treatment). (E) Representative images of Prosense680 signal intensity. (F-G) Normalized osteophyte volume quantification (n=8 per treatment) and (G) representative μCT images. Dashed red lines outline osteophytes. (H-I) Histopathologic scoring of total joint PTOA severity (H) and total joint synovitis severity (I) (n=7-8 per treatment). (J-K) Representative sagittal histological images for PTOA score (J) and synovitis score (K) of ACLR joints in Vehicle (top) and PolySA (bottom). Yellow arrows - osteophyte, green arrows - structural damage, pink arrows - subchondral bone thickening, purple arrows - fibrosis. Scale bar = 500μm. Statistical analyses for B-I used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.

Journal: bioRxiv

Article Title: Acute immune modulation with poly-salicylic acid particles ameliorates pain and structural damage in post-traumatic osteoarthritis

doi: 10.1101/2025.11.21.689790

Figure Lengend Snippet: (A) Experimental schematic indicating the timing of two PolySA or Vehicle injections following ACLR, with endpoint harvests performed at 28d post-ACLR. (B-C) Knee withdrawal threshold of the ACLR limb (B) and ACLR/contralateral knee withdrawal threshold ratio (C) at baseline and 28d post-ACLR (n=8 per treatment). (D) Signal intensity quantification from ProSense680 NIR molecular imaging in the ACLR limb at 28d (n=8 per treatment). (E) Representative images of Prosense680 signal intensity. (F-G) Normalized osteophyte volume quantification (n=8 per treatment) and (G) representative μCT images. Dashed red lines outline osteophytes. (H-I) Histopathologic scoring of total joint PTOA severity (H) and total joint synovitis severity (I) (n=7-8 per treatment). (J-K) Representative sagittal histological images for PTOA score (J) and synovitis score (K) of ACLR joints in Vehicle (top) and PolySA (bottom). Yellow arrows - osteophyte, green arrows - structural damage, pink arrows - subchondral bone thickening, purple arrows - fibrosis. Scale bar = 500μm. Statistical analyses for B-I used linear mixed effect models. For knee withdrawal threshold, the contralateral limb was considered as a covariate for the ACLR limb and vice versa. Graphs show the mean +/- SEM.

Article Snippet: At harvest, either 7d or 28d post-ACLR, mice were euthanized, skinned, and NIR imaging (Pearl Impulse, LI-COR, Lincoln, NE, United States) was performed (7d: n=4 per sex/treatment, 28d: n=8 females per treatment).

Techniques: Imaging